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Table 3 miRNA assay control experiments - overview

From: Chemokine receptor CCR6 expression is regulated by miR-518a-5p in colorectal cancer cells

Control experiment Type Example
Positive control miRNA miRNA that is known to downregulate its target gene miR-1 Mimic (targeting TWF1)
Negative control miRNA (NK) A nonsilencing miRNA with no homology to any known mammalian gene microRNA Mimic negative control (Applied Biosystems)
Mock transfection control Control experiment where cells go through the transfection process without addition of miRNA  
Untransfected cells control Control experiment where gene expression analysis is carried out on cells that have not gone through the transfection process