Table 3 miRNA assay control experiments - overview
From: Chemokine receptor CCR6 expression is regulated by miR-518a-5p in colorectal cancer cells
Control experiment | Type | Example |
|---|---|---|
Positive control miRNA | miRNA that is known to downregulate its target gene | miR-1 Mimic (targeting TWF1) |
Negative control miRNA (NK) | A nonsilencing miRNA with no homology to any known mammalian gene | microRNA Mimic negative control (Applied Biosystems) |
Mock transfection control | Control experiment where cells go through the transfection process without addition of miRNA | |
Untransfected cells control | Control experiment where gene expression analysis is carried out on cells that have not gone through the transfection process |