Luciferase activity after site-directed mutagenesis in miR-518a-5p transfected 293 T cells. Luciferase activity in 293 T cells cotransfected with Luc CCR6, Luc No CCR6 and Luc CCR6 Mut BS1, Luc CCR6 Mut BS2 and Luc CCR6 Mut BS1 + 2, respectively, and miR-518a-5p relative to NK miR mimic transfected cells. Luciferase activities are presented as mean +/- SEM (n = 10). Luciferase activity after Site Directed Mutagenesis of the 3′UTR of CCR6 in the miR-518a-5p Seed Sequences was significantly higher in all three mutated plasmids with respect to the Luc-CCR6 vector transfected cells *(P < 0.05). Fold decrease below 1 indicates luciferase down-regulation related to NK miR mimic transfected cells.