Figure 4From: Chemokine receptor CCR6 expression is regulated by miR-518a-5p in colorectal cancer cells Dual luciferase reporter assay system with mutated 3′UTR of CCR6. Site-directed mutagenesis was introduced into the 2 seed sequences of hsa-miR-518a-5p in positions 222–228 and 1773–1779 in the 3′UTR of CCR6 resulting in three mutated luciferase expression vectors designated (A) Luc CCR6 Mut BS1, (B) Luc CCR6 Mut BS2 and (C) Luc CCR6 Mut BS1 + 2, respectively.Back to article page