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Figure 5 | Journal of Translational Medicine

Figure 5

From: MicroRNA-362 induces cell proliferation and apoptosis resistance in gastric cancer by activation of NF-κB signaling

Figure 5

MiR-362 suppresses CYLD expression by directly targeting the CYLD 3′-UTR. (A) Predicted miR-362 target sequence in the 3′-UTR of CYLD (CYLD -3′UTR) and mutant containing two mutated nucleotides in the CYLD 3′-UTR (CYLD-3′UTR-mut). (B) Western blot of CYLD expression in cells into which miR-362 had been transduced or inhibited (miR-362-in). α-Tubulin served as the loading control. (C) Western blot of GFP expression in cells. α-Tubulin served as the loading control. (D) Luciferase assay of pGL3-CYLD-3′UTR reporter in cells into which miR-362 had been transduced or inhibited. (E) Luciferase assay of cells transfected with pGL3-CYLD-3′UTR or pGL3-CYLD-3′UTR-mut reporter. (F) Luciferase reported NF-κB activity in cells transfected with Scramble, CYLD siRNA(s), or CYLD siRNA(s) plus miR-362. (G) Luciferase reported NF-κB activity in cells transfected with miR-362 inhibitor or miR-362 inhibitor plus CYLD siRNA(s). Bars denote the mean ± SD of three independent experiments. *P < 0.05. NC, Negative control.

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