Figure 3

MiR-362 inhibition suppresses cell proliferation and induces apoptosis of gastric cancer cells. (A) MTT assay revealing that miR-362 inhibition suppressed the growth of BGC-823 and SGC-7901 gastric cancer cells. (B) Representative micrographs (left) and quantification (right) of crystal violet–stained cell colonies. (C) Flow cytometric analysis of gastric cancer cells transduced with negative control (NC) or miR-362 inhibitor (miR-362-in). (D) Annexin V–FITC/PI staining of cells treated with 20 μM cisplatin for 24 h. (E) Representative micrographs (left) and quantification of TUNEL-positive cells in cells treated with 20 μM cisplatin for 36 h. Bars denote the mean of three independent experiments. *P < 0.05.