Figure 2

MiR-362 upregulation promotes cell proliferation and induces apoptosis resistance in gastric cancer cells. (A) MTT assay revealing that miR-362 upregulation induced growth in BGC-823 and SGC-7901 gastric cancer cells. (B) Representative micrographs (left) and quantification (right) of crystal violet–stained cell colonies. (C) Flow cytometric analysis of gastric cancer cells transduced with vector or miR-362. (D) Annexin V–FITC/PI staining of cells treated with 20 μM cisplatin for 24 h. (E) Representative micrographs (left) and quantification of TUNEL-positive cells in cells treated with 20 μM cisplatin for 36 h. Bars denote the mean of three independent experiments. *P < 0.05.