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Table 3 Antigenic analysis by flow cytometry of MSC expanded with control PL and PI-PL preparations from P1 to P3

From: Pathogen-free, plasma-poor platelet lysate and expansion of human mesenchymal stem cells

MSC PL or PI-PL CD105+% CD90+% CD71+% CD73+%
P1@ P3* P1§ P3# P1+ P3^ P1° P3_
MSC-1 PL1 92 98 92 98 95 99 95 99
PI-PL1 94 99 92 99 95 100 95 100
MSC-3 PL1 93 98 94 98 95 99 95 99
PI-PL2 93 95 90 90 95 98 95 98
MSC-6 PL1 94 96 93 99 94 99 94 99
PI-PL3 91 98 94 98 95 99 95 99
MSC-3 PL2 94 97 91 97 92 98 92 98
PI-PL1 94 97 95 98 95 99 95 99
MSC-4 PL2 93 96 92 98 94 99 90 99
PI-PL2 93 98 92 97 94 99 94 99
MSC-7 PL2 93 96 91 99 92 98 95 99
PI-PL3 92 96 94 98 95 98 95 98
MSC-5 PL3 90 96 90 98 94 99 94 99
PI-PL1 93 98 92 98 94 99 94 99
MSC-6 PL3 93 97 95 99 90 98 92 99
PI-PL2 92 98 94 99 94 99 94 99
MSC-8 PL3 92 99 94 98 95 99 93 96
PI-PL 3 90 99 92 97 94 99 90 98
  1. PL platelet lysate, PI-PL pathogen inactivated platelet lysate, MSC mesenchymal stem cells, P1 passage 1 of culture, P3 passage 3 of culture; CD34 and CD45 expression was consitently lower than 5% in all cultures at any time point. Each antigen is expressed as percentage of positive cells detected at P1 and P3 in each flow cytometric analysis performed on different MSC populations (1,3,4,5,6,7,8) which were grown in the presence of the indicated PL or PI-PL. @p = 0.833, *p = 0.402, §p = 0.615, #p = 0.254, +p = 0.095, ^p = 0.346, °p = 0.287 and _p = 0.512 at paired two-tailed Student’s T test between antigen expression in cultures supported by control PL and PI-PL lots.
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