Pathogen-free, plasma-poor platelet lysate and expansion of human mesenchymal stem cells

Table 3 Antigenic analysis by flow cytometry of MSC expanded with control PL and PI-PL preparations from P1 to P3

MSC	PL or PI-PL	CD105+%		CD90+%		CD71+%		CD73+%
MSC	PL or PI-PL	P1^@	P3*	P1^§	P3^#	P1⁺	P3^{^}	P1°	P3_
MSC-1	PL1	92	98	92	98	95	99	95	99
MSC-1	PI-PL1	94	99	92	99	95	100	95	100
MSC-3	PL1	93	98	94	98	95	99	95	99
MSC-3	PI-PL2	93	95	90	90	95	98	95	98
MSC-6	PL1	94	96	93	99	94	99	94	99
MSC-6	PI-PL3	91	98	94	98	95	99	95	99
MSC-3	PL2	94	97	91	97	92	98	92	98
MSC-3	PI-PL1	94	97	95	98	95	99	95	99
MSC-4	PL2	93	96	92	98	94	99	90	99
MSC-4	PI-PL2	93	98	92	97	94	99	94	99
MSC-7	PL2	93	96	91	99	92	98	95	99
MSC-7	PI-PL3	92	96	94	98	95	98	95	98
MSC-5	PL3	90	96	90	98	94	99	94	99
MSC-5	PI-PL1	93	98	92	98	94	99	94	99
MSC-6	PL3	93	97	95	99	90	98	92	99
MSC-6	PI-PL2	92	98	94	99	94	99	94	99
MSC-8	PL3	92	99	94	98	95	99	93	96
MSC-8	PI-PL 3	90	99	92	97	94	99	90	98

PL platelet lysate, PI-PL pathogen inactivated platelet lysate, MSC mesenchymal stem cells, P1 passage 1 of culture, P3 passage 3 of culture; CD34 and CD45 expression was consitently lower than 5% in all cultures at any time point. Each antigen is expressed as percentage of positive cells detected at P1 and P3 in each flow cytometric analysis performed on different MSC populations (1,3,4,5,6,7,8) which were grown in the presence of the indicated PL or PI-PL. ^@p = 0.833, *p = 0.402, ^§p = 0.615, ^#p = 0.254, ⁺p = 0.095, ^{^}p = 0.346, °p = 0.287 and _p = 0.512 at paired two-tailed Student’s T test between antigen expression in cultures supported by control PL and PI-PL lots.

ISSN: 1479-5876