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Table 2 Comparisons between control PL and PI-PL lots to support expansion of the same MSC populations

From: Pathogen-free, plasma-poor platelet lysate and expansion of human mesenchymal stem cells

MSC PL or PI-PL P1 P3 Fold Expansion* Doubling Time (hours)°
Day to reach MSCx106 § Day to reach MSCx106 #  
MSC-1 PL1 16 0.847 27 142 168 35.7
PI-PL1 16 0.765 27 120 157 36.2
MSC-3 PL1 16 0.572 31 182 319 43.3
PI-PL2 16 0.564 31 177 313 43.4
MSC-6 PL1 15 1.150 27 116 97 43.3
PI-PL3 15 1.264 27 162 128 41.1
MSC-3 PL2 15 0.933 32 204 219 52.5
PI-PL1 15 0.950 32 212 223 52.3
MSC-4 PL2 13 0.560 31 101 181 57.6
PI-PL2 13 0.638 31 77 177 62.5
MSC-7 PL2 15 0.570 27 46 81 45.5
PI-PL3 15 0.563 27 83 148 40
MSC-5 PL3 12 0.940 30 144 154 59.5
PI-PL1 12 0.948 30 117 123 62.2
MSC-6 PL3 13 1.056 31 100 95 65.8
PI-PL2 13 0.899 31 103 115 63.2
MSC-8 PL3 13 0.970 32 109 112 66.9
PI-PL 3 13 1.155 32 113 98 68.9
  1. PL platelet lysate, PI-PL pathogen inactivated platelet lysate, MSC mesenchymal stem cells, P1 passage 1 of culture, P3 passage 3 of culture; different MSC populations (1,3,4,5,6,7,8) were obtained by the culture of a starting number of 5 × 106 mononuclear cells from as many bone marrow samples.
  2. §p = 0.640 and #p = 0.802 at paired two-tailed Student’s T test between MSC growth promoted by control PL and PI-PL lots. *fold expansion at P3 as compared to cells obtained at P1, p = 0.541 at paired two-tailed Student’s T test between MSC expansion promoted by control PL and PI-PL lots. °doubling time measured as hours between P1 and P3 for each MSC population, p = 0.975 at paired two-tailed Student’s T test between control PL and PI-PL lots.
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