Pathogen-free, plasma-poor platelet lysate and expansion of human mesenchymal stem cells

Table 2 Comparisons between control PL and PI-PL lots to support expansion of the same MSC populations

MSC	PL or PI-PL	P1		P3		Fold Expansion*	Doubling Time (hours)°
MSC	PL or PI-PL	Day to reach	MSCx10⁶ ^§	Day to reach	MSCx10⁶ ^#		Doubling Time (hours)°
MSC-1	PL1	16	0.847	27	142	168	35.7
MSC-1	PI-PL1	16	0.765	27	120	157	36.2
MSC-3	PL1	16	0.572	31	182	319	43.3
MSC-3	PI-PL2	16	0.564	31	177	313	43.4
MSC-6	PL1	15	1.150	27	116	97	43.3
MSC-6	PI-PL3	15	1.264	27	162	128	41.1
MSC-3	PL2	15	0.933	32	204	219	52.5
MSC-3	PI-PL1	15	0.950	32	212	223	52.3
MSC-4	PL2	13	0.560	31	101	181	57.6
MSC-4	PI-PL2	13	0.638	31	77	177	62.5
MSC-7	PL2	15	0.570	27	46	81	45.5
MSC-7	PI-PL3	15	0.563	27	83	148	40
MSC-5	PL3	12	0.940	30	144	154	59.5
MSC-5	PI-PL1	12	0.948	30	117	123	62.2
MSC-6	PL3	13	1.056	31	100	95	65.8
MSC-6	PI-PL2	13	0.899	31	103	115	63.2
MSC-8	PL3	13	0.970	32	109	112	66.9
MSC-8	PI-PL 3	13	1.155	32	113	98	68.9

PL platelet lysate, PI-PL pathogen inactivated platelet lysate, MSC mesenchymal stem cells, P1 passage 1 of culture, P3 passage 3 of culture; different MSC populations (1,3,4,5,6,7,8) were obtained by the culture of a starting number of 5 × 10⁶ mononuclear cells from as many bone marrow samples.
^§p = 0.640 and ^#p = 0.802 at paired two-tailed Student’s T test between MSC growth promoted by control PL and PI-PL lots. *fold expansion at P3 as compared to cells obtained at P1, p = 0.541 at paired two-tailed Student’s T test between MSC expansion promoted by control PL and PI-PL lots. °doubling time measured as hours between P1 and P3 for each MSC population, p = 0.975 at paired two-tailed Student’s T test between control PL and PI-PL lots.

ISSN: 1479-5876