Figure 8
From: Pathogen-free, plasma-poor platelet lysate and expansion of human mesenchymal stem cells
Comparison of growth factor concentration and growth promoting-ability between PL18 and PI-PL18. PL18 and PI-PL18 were generated by the same initial lot (omitting PI step in PL18 preparation) and were compared to assess the impact of PI on growth factors and MSC growth in a head to head comparison. a) growth factor concentrations were evaluated in triplicates for both samples and compared by two-tailed paired Student’s T test which shows no significant differences; b) MSC170, MSC180, MSC082 MSC11 populations were grown in parallel in the presence of either PL18 or PI-PL18; cell growth was expressed as doubling time (DT) between passage 1 (P1) and passage 2 (P2). Statistical analysis by two-tailed paired Student’s T test between PL18- or PI-PL18-induced growth on different MSC populations shows no signficance in the direct comparison.