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Figure 6 | Journal of Translational Medicine

Figure 6

From: Survivin-responsive conditionally replicating adenovirus kills rhabdomyosarcoma stem cells more efficiently than their progeny

Figure 6

Viral replication of Surv.m-CRA and cytotoxicity against sorted FGFR3-positive and FGFR3- negative cells. (A) Growth curves of sorted cells. Whereas FGFR3-positive cells proliferated somewhat more rapidly than FGFR3-negative cells, the difference was not drastic. *, P < 0.05. (B) Representative images of phase-contrast and the fluorescence-microscopic pictures 5 days after infection with Surv.m-CRA, Ad.dE1.3, or no virus. Whereas viral replication and cytopathic effects of Surv.m-CRA were prominent in both groups, the proportion of remaining cells 5 days after Surv.m-CRA infection at an MOI of 1 was lower in sorted FGFR3-positive cells than in sorted FGFR3-negative cells. (C) Cell viability was determined by WST-8 assay 3 or 5 days after infection. Cytotoxic effects of Surv.m-CRA were statistically significantly higher than those of Ad.dE1.3 solely in the sorted FGFR3-positive cells. *, P < 0.05; **, P < 0.005 between Surv.m-CRA and either Ad.dE1.3 or no virus.

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