Figure 5
Viral replication of Surv.m -CRA and cytotoxicity against KYM-1 cells cultured in serum-minus and serum-plus media. (A) Schematic representation of the construction of Surv.m-CRA. (B) Representative images of phase-contrast and fluorescence-microscopic pictures 5 days after infection with Surv.m-CRA, Ad.dE1.3, or no virus (none) of KYM-1 cells cultured in serum-minus or serum-plus media. Viral replication and cytopathic effects were prominent in both groups after Surv.m-CRA infection. (C) Cell viability was determined by WST-8 assay 3 or 5 days after infection.*, P < 0.05. **, P < 0.005. Surv.m-CRA efficiently replicated and potently induced cell death in both cell fractions cultured in serum-minus and serum-plus media.