Survivin mRNA expressions and survivin promoter activity. (A, B) Expression levels of survivin mRNA under serum-minus and serum-plus conditions (A) and in sorted FGFR3-positive and FGFR3-negative cells (B) were measured by real-time qRT-PCR. The HPRT gene was amplified as an internal control. (C) Flow-cytometric analysis to detect LacZ-expressing cells 48 h after Ad.Surv-LacZ or Ad.RSV-LacZ infection at MOI of 30 demonstrated the activities of the survivin and control RSV promoters in individual cells. (D, E) β-galactosidase activities in cells cultured in serum-minus or serum-plus media (D) and in sorted FGFR3-positive and FGFR3-negative cells (E) were measured after the same infection described in (C). *, P < 0.05.