Local hF.IX expression and CD8 infiltration in LS mice. Skeletal muscle from LS mice injected i.m. with 1011 vg ss or scAAV1 (n = 4/group) was harvested, cryosectioned, and stained for hF.IX (red) and CD8 (green). Nuclei were visualized with DAPI (blue). Four weeks post-injection, tissue was harvested from mice injected with ssAAV1 (A-B) or scAAV1 (C-D). Representative images from two mice are shown for each condition. The scale bar represents 100 μm. Results are representative of at least two independent experiments.