Figure 4

BBR inhibits TGF-β1-induced EMT markers and transcription factors. (A) Protein level of E-cadherin was measured by Western blotting assay after A549 cells were treated with different concentrations of BBR for 48 h. (B) Protein level of Snail1and Slug were measured by Western blotting assay after A549 cells were treated with different concentration of BBR for 48 h. (C) Protein level of MMP2, MMP-9, p-Smad2/3 and Smad2/3 were measured by Western blotting assay after A549 cells were treated with different concentration of BBR for 48 h. (D) mRNA level of E-cadherin and Vimentin was measured by real-time PCR assay after A549 cells were treated with 20 μM BBR for 24 h. Values are expressed as mean ± SD of three experiments.*P <0.05, **P <0.01, as compared with control group or TGF-β1 stimulation group.