Figure 4

p38MAPK and NF-κB signaling are necessary for Hp91-mediated IL-6 secretion by dendritic cells. Immature human DCs were pre-treated for 30 min with DMSO control (D), p38 inhibitor SB203580 at 5 μM (SB5) or 20 μM (SB20), MEK1 inhibitor PD98059 (PD) at 20 μM, or NF-κB inhibitor TPCK at 20 μM prior to exposure to Hp91. Cell culture supernatants from the different groups were collected after 48 h and analyzed for the presence of IL-6 by ELISA. Results are mean (±SEM) for N = 5. *p < 0.05; Student’s t-test.