Figure 11From: Association of cancer metabolism-related proteins with oral carcinogenesis – indications for chemoprevention and metabolic sensitizing of oral squamous cell carcinoma? Western blot analysis in normal tissue, BICR3 and BICR56 OSCC cell lines. Western Blot of HK 2, IGF-R1β, PFK-1, SDHA, ATP synthase, LDHA, and SDHB in BICR3 and BICR56 OSCC cell lines confirm immunohistochemical staining specifity of antibodies (left panel, A). Western blot analysis shows increased HK 2 (~102 kDa), IGF-R1β (~97 kDa), PFK-1 (~85 kDa), SDHA (~70 kDa), ATP synthase (~53 kDa), LDHA (~37 kDa), and SDHB (~32 kDa) expression compared to normal tissue. GAPDH (loading control) is shown as a band of approximately 35 kDa. Densitometric quantification (B) of western blot protein bands (pixel total) is given in the right panel (B). IGF-1R, insulin-like growth factor-1 receptor; HK 2, hexokinase 2; PFK-1, phosphofructokinase-1; LDHA, lactate dehydrogenase A; SDH, succinate dehydrogenase; GAPDH, glycerinaldehyd-3-phosphat-dehydrogenase; N.T., normal tissue.Back to article page