Figure 10

In situ detection of DNA fragmentation by TUNEL-staining in OSCC. In metabolic highly active regions as demonstrated by markers IGF-R1β, HK 2, TKTL1, PFK-1, LDHA, SDHA, SDHB, and ATP synthase cancer cells do not undergo apoptosis (serial section, asterisk, A). In contrast, tumor-infiltrating leucocytes adjacent to the tumor demonstrate increased apoptotic activity (arrow, B). Brown chromogen color (3,3′-Diaminobenzidine) indicates positive staining, the blue color shows the nuclear counterstaining by hematoxylin. Original magnification: x200-fold. TUNEL, terminaldeoxynucleotidyl transferase-mediated deoxyuridinetriphosphate nick-end labeling.