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Figure 4 | Journal of Translational Medicine

Figure 4

From: In vitro blood cell responsiveness to IFN-α predicts clinical response independently of IL28B in hepatitis C virus genotype 1 infected patients

Figure 4

ISG expression, stratified based on IL28B genotype, is similar between G1 and G3 infected patients. PBMCs were isolated from HCV infected patients prior to them commencing IFN-α therapy and were stimulated in vitro with 1000 IU of IFN-α for 4 h. qRT-PCR was used to quantify endogenous and IFN-α stimulated expression of ISGs. ISG expression was stratified based on rs12979860 genotype and (A) endogenous and (B) IFN-α stimulated ISG expression was measured. All gene expression was normalised to expression of the internal control gene RPS15 and expressed on a log scale. Each dot represents one sample and median gene expression is shown. Data were analysed using Wilcoxon matched-pairs rank test and Mann–Whitney U-tests.

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