Table 3 Selection of VHH antibodies against HCC
Number of clone | FR1 | CDR1 | FR2 | CDR2 |
|---|---|---|---|---|
3-2 | MADVQLQASGGGLVQAGGSLRLSCAAS | GSIVSIND | MGWYRQAPGKQRDLVAL | ITRGGNT |
3-24 | MADVQLQASGGGLVQPGGSLRLSCAVS | GTNFRLND | MAWYRQPPEKRRELVAL | ITGGGNT |
3-30 | MADVQLQASGGGLVQAGGSLRLSCAAS | GSIASIHD | MGWYRQTPGKQRDLVAL | ITRGGNT |
3-33 | MAEVQLQASGGGLVQPGGSLRLSCAAS | RMVIRTFSGAD | MGWYRQISRNQRELVAL | ITSGGNT |
4-5 | MAEVQLQASGGGLVQPGGSLRLSCAA | RMVFSTFSGAD | MGWYRQISGNQRELVAL | ITSGGNT |
4-8 | MAEVQLQASGGGLVQPGGSLRLSCAAS | GSIFSIND | MGWYRQAPGKQRELVAF | ITRGGNTH |
Number of clone | FR3 | CDR3 | FR4 | |
3-2 | NYADSVKGRFTISRDNAKSTVYLQMNNLKPEDTAVYYC | ATLTRPAYW | GQGTLVTVSSGR | |
3-24 | SYADSVKDRFTISRDNIQRTLYLQMNSLKPEDTAVYYC | TTQRSGRQYW | GKGTHVTVSSGR | |
3-30 | NYADSVKGRFTISRDNAKSTVYLQMNSLKPEDTAVYYC | ATLTRPAYW | GQGTLVTVSSGR | |
3-33 | NYTDSVKGRFTISRDNAKGTLYLQMSNLKPEDTAHYYC | AKISFTGPHRW | GQGTQVTVSSGR | |
4-5 | NYTDSVKGRFTISRDNAKGTLYLQMSSLKPEDTAHYYC | AKISRTTPHYW | GQGTQVTVSSGR | |
4-8 | HYADSAKGRFTISRDNAKNTLYLQMNSLKPEDTAVYYC | NTVNTRTRSW | GQGTQVTVSSGR | |