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Figure 4 | Journal of Translational Medicine

Figure 4

From: Aurora-A contributes to cisplatin resistance and lymphatic metastasis in non-small cell lung cancer and predicts poor prognosis

Figure 4

Aurora-A overexpression correlates with cisplatin resistance in vitro. (A) Protein level of Aurora-A was detected in A549 and A549/DDP cells or in H460 and H460/DDP cells by western blotting. Equal loading of protein was determined by GAPDH. (B) The staining of Aurora-A by Immunofluorescence in A549 and A549/DDP cells. Red: Aurora-A; Green: Tubulin; Blue: DAPI. (C) A549 and H460 cells were transfected with an expression vector for Aurora-A or control (vector) and detected by western blotting (left panel). Cells were treated with indicated concentrations of DDP for 24 h and subjected to MTT assay (right panel). (D) Aurora-A was overexpressed in A549 cells through lentivirus-mediated gene transfer. Two days after transfection, cells were selected by using 2 μg/ml puromycin. Cells were harvested for western blotting analysis and subjected to colony formation assay with incubation of DDP (2, 5 μM) or vehicle (0 μM) (n = 3), **p < 0.01, two-tailed Student’s t-tests.

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