miR-208 promotes ESCC progression by inhibiting SOX6. A. The expression levels of SOX6 in Kyse30-miR-208-inhibitor and Kyse410-miR-208-inhibitor cells that were transfected with SOX6-siRNA, as measured by western blotting. α-Tubulin served as the loading control. B. The growth rates in SOX6-silenced cells as indicated by the MTT assay. C. Representative micrographs (left) and quantification (right) of crystal violet stained cell colonies formed by indicated ESCC cell lines 10 days after inoculation. D. Representative images (left) and quantification (right) of colony numbers of indicated cells determined by an anchorage-independent growth assay. Colonies larger than 0.1 mm in diameter were scored. Error bars represent mean ± SD from three independent experiments. *P < 0.05.