Inhibition of miR-208 reduces the proliferation of ESCC. A. Real-time PCR analysis of miR-208 expression in Kyse30 and Kyse410 cells transfected with miR-208 inhibitor. B. The proliferation ability of ESCC cells Kyse30 and Kyse410 transfected with miR-208 inhibitor or NC measured by the MTT assay. C. Representative micrographs (left) and quantification (right) of crystal violet stained cell colonies formed by indicated ESCC cell lines, 10 days after inoculation. D. The tumorigenicity of ESCC cells Kyse30 and Kyse410 transfected with miR-208 inhibitor or NC measured, as by an anchorage-independent growth ability assay. Colonies larger than 0.1 mm were scored. E. Flow cytometry analysis of indicated ESCC cells transfected with miR-208-inhibitor or NC. Each bar represents the mean ± SD of three independent experiments. *P <0.05.