Expression of miR-208 is increased in ESCC cell lines and tissues. A. Real-time PCR analysis of miR-208 expression in normal esophageal epithelial cells (NEEC) and esophageal squamous carcinoma cells, including Kyse140, Kyse30, Kyse510, Kyse520, Eca109, TE-1, Kyse410, Kyse180, EC18, HKESC1 and 108CA. Transcript levels were normalized using U6 expression. B. The expression of miR-208 was examined in 10 paired cancerous tissues (T) and their adjacent noncancerous esophageal tissues (ANT). The average miR-208 expression was normalized using U6 expression. Each bar represents the mean ± SD of three independent experiments. *P <0.05.