Ipilimumab altered the T cell-subpopulation profile in expanded ATC. ( a ): ATC expanded with ipilimumab had a decreased CD4/8 ratio (n = 11) ( left ). The panel summarizes 11 experiments (n = 11); the bars in the boxes are medians while + symbols represent means. One representative flow cytometry analysis is shown ( right ). ( b ): The proportion of CD4 and CD8 T cells were determined in the presence or absence of ipilimumab. Control and ipilimumab groups in each of 11 experiments are connected with a trend line. ( c ): The expanded 8 ATC products were analyzed by flow cytometry to show the change in the Treg population (CD3+CD4+CD25high/+CD127low/-). The bars in the boxes are medians while + symbols represent means. ( d ): NKT cell population (CD3+CD56+) in the expanded ATC products is shown. Each bar represents a mean with standard error of eight experiments (n = 8). Each bar represents a mean ± SE of 8 experiments, and paired, two-tailed t test was performed.