Soluble CD147 and MMP-2 levels in the serum of HCC patients and healthy individuals. (A) Serum samples of four HCC patients were immunoprecipitated for CD147 extracellular domain by HAb18 and immunoblotted for CD147 intracellular domain by C-19 antibody. (B) Serum samples of a healthy individual and number 4 patient with HCC were immunoprecipitated for CD147 intracellular domain by C-19 antibody, and the samples containing bait:prey complexes and the flow-through fractions were detected with HAb18 antibody, respectively. (C) Serum levels of soluble CD147 and MMP-2 were determined by ELISA assay. Bars indicated mean values in each group. (D) Expression of CD147 and MMP-2 in human HCC tissues assessed by immunohistochemistry (×400). Representative positive and negative samples were shown. (E) Correlation of serum soluble CD147 level with membrane-bound CD147 (n = 26) and MMP-2 expression (n = 15) in HCC tissues. (F) Comparison of serum soluble CD147 and serum MMP-2 in HCC patients with different AFP levels. Serum AFP levels were examined by electrochemiluminescence analyzer in Xijing Hospital of Fourth Military Medical University. Bars indicated mean values in each group.