Involvement of ERK, FAK, and PI3K/Akt pathways in upregulation of MMP-2 driven by soluble CD147. SMMC-7721 cells were cultured for 30 min (A) and 12 h (B) with 10 μg/ml of E-CD147ECD. CD147 and MMP-2 data were expressed as means ± SEM of three independent determinations of CD147 or MMP-2/α-tubulin ratio. (C) SMMC-7721 cells were treated with E-CD147ECD (10 μg/ml) for 12 h with or without pre-treatment with ERK inhibitor (U0126), FAK inhibitor (FAK inhibitor 14), PI3K inhibitor (LY294002), or combined inhibitors. In A and C, the expression levels of phospho-ERK1/2, ERK1/2, phospho-FAK, FAK, phospho-Akt, Akt, phospho-EGFR, and EGFR were detected by western blotting. Quantitative analysis of phosphorylated fraction relative to the total fraction was shown. Mean values for control groups were normalized to 1. *P < 0.05, **P < 0.01, ***P < 0.001.