A theoretical timeline for myocardial infarction: immunohistochemical evaluation and western blot quantification for Interleukin-15 and Monocyte chemotactic protein-1 as very early markers

Table 1 Semi-quantitative evaluation and statistical analysis of the immunohistochemical findings and gradation of the immunohistochemical reaction in the heart samples

Antibody	Group A (≤4-6 hours from the onset of ischemia related symptoms)	Group B (≥6-8 hours from the onset of ischemic symptoms and signs)	Group C controls	Significant level	Significance levels¹
CD15	+	+++	-	A vs C	**
				B vs C	***
				A vs B	**
IL-1β	+	+++	−	A vs C	**
				B vs C	***
				A vs B	**
IL-6	+	+++	-	A vs C	**
				B vs C	***
				A vs B	**
TNF-α	+	+++	-	A vs C	**
				B vs C	***
				A vs B	**
IL-15	+++	+	−	A vs C	***
				B vs C	**
				A vs B	**
IL-8	++	+++	−	A vs C	**
				B vs C	***
				A vs B	*
MCP-1	+++	+	-	A vs C	***
				B vs C	**
				A vs B	**
ICAM-1	+	++	−	A vs C	*
				B vs C	**
				A vs B	*
CD18	-	-	−	A vs C	ns
				B vs C	ns
				A vs B	ns
Troponin C	+	+++	_	A vs C	**
				B vs C	***
				A vs B	**
Troponin I	+	+++	_	A vs C	**
				B vs C	***
				A vs B	**
Tryptase	+	+++	−	A vs C	**
Tryptase	+	+++	−	B vs C	***
				A vs B	**

¹Intensity of immunopositivity was assessed semiquantitatively in the scale 0–4 as follows: 1. (0): not expressed, 2. (+): isolated and disseminated expression, 3. (++): expression in widespread foci, 4. (+++): widespread expression.
NS: p > 0.05; *: p < 0.05; **: p < 0.01; ***: p < 0.001.

ISSN: 1479-5876