CD4+CD25-CD69+ T cells might not display immune regulatory function via cytokine secretion. (a). The frequency of CD4+CD25-CD69+ T cells in group B was significantly higher than those in group A and healthy donors. (b). No significant difference of cytokine (IL-2, IL-10 and TGF-β) level was seen among donors and patients of group A & B before PMA and ionomycin stimulation. (c). There was no obvious difference of cytokine secretion between group A and group B after PMA and ionomycin stimulation.