Figure 5

Increased 4-hydroxynonenal (HNE)-protein adducts was not reduced by UDCA administration. C57BL/6 mice were fed with either SD or HFSD for 24 weeks upon which mice on HFSD were divided into two groups, and fed with either 20 mg/kg UDCA diluted in 0.78% Tween-80 or 0.78% Tween-80 only. Mice fed with SD were also given 0.78% Tween-80 as vehicle. A quantitative OxiSelect HNE-His Adduct enzyme-linked immunosorbent assay kit was used for determination of HNE-protein adducts, which is the consequence of oxidative stress. Feeding HFSD significantly produced HNE adducts, and this was not reduced by UDCA administration.