Atherogenesis in ApoE-/- model after 30 days of CS exposure. (A). Concentration of lipoproteins in serum of ApoE-/- mice exposed to sham or to CS for 30 days. (B). Plaque area in the aortic arch of sham and CS-exposed mice. (C/D). Representative images of the aortic arch of 12 week old Apo E-/- mice after the end of the exposure period. (E/F). Representative images of H&E stained cross sections of the aortic roots of Apo E-/- mice at the end of the exposure period. Atherosclerotic lesions are encircled. (G). Immunohistochemical staining with an anti-Mac-3 antibody revealed that atherosclerotic lesions from the aortic root of a CS-exposed animal displayed significant presence of macrophages.