Quantitative measurement of alterations in DNA damage repair (DDR) pathways using single cell network profiling (SCNP)

Table 1 T-Test P-values comparing DDR readouts between BRCA1 +/- vs. BRCA1 +/+ cell lines

Treatment	AZD			AZD			AZD + TMZ			AZD + TMZ
Timepoint:	48 h			72 h			48 h			72 h
Population:	All cells	Cyclin A2-	Cyclin A2+	All cells	Cyclin A2-	Cyclin A2+	All cells	Cyclin A2-	Cyclin A2+	All cells	Cyclin A2-	Cyclin A2+
Stain:
p-H2AX	0.536	0.119	0.012	0.558	0.151	0.039	0.673	0.172	0.014	0.509	0.272	0.011
p-RPA2	0.545	0.372	0.545	0.599	0.442	0.229	0.875	0.445	0.916	0.900	0.767	0.859
p-DNA-PKcs	0.398	0.419	0.957	0.597	0.469	0.336	0.776	0.952	0.894	0.758	0.562	0.297
p21	0.315	0.413	0.132	0.645	0.737	0.203	0.693	0.708	0.364	0.778	0.706	0.452
p-ATM	0.636	0.848	0.390	0.940	0.693	0.228	0.746	0.967	0.736	0.813	0.920	0.694
p-BRCA1	0.754	0.954	0.608	0.505	0.872	0.721	0.858	0.839	0.756	0.684	0.970	0.860

Statistically significant p-values (p < .05) for stratifying BRCA1+/- vs. BRCA1+/+cell lines are shown in bold.

ISSN: 1479-5876