Figure 8

Genotoxin effects on CyclinA2 subets of leukemic cells or lymphocytes from myeloid growth factor stimulated AML samples. Shown are flow plot examples of CyclinA2 vs. p-H2AX for leukemic AML cells (left) or normal lympohcytes (right) gated (see Materials and methods) from 5 AML samples treated with etoposide (30 μg/mL), PARP inhibitor AZD2281 (6 μg/mL), temozolomide (10 μg/mL), or the combination of AZD2281 + temozolomide (6 μg/mL + 10 μg/mL) for 6 hr. While leukemic CyclinA2+ cells show induced p-H2AX from all agents tested, leukemic and lymphocytic CyclinA2- cells only demonstrate induced p-H2AX after etoposide treatment.