Figure 4From: Metallothionein III (MT3) is a putative tumor suppressor gene that is frequently inactivated in pediatric acute myeloid leukemia by promoter hypermethylation Overexpression of MT3 inhibited proliferation an induced apoptosis in leukemia cells. (A) Transfection with MT3 lentivirus PLVX-MT3 significantly upregulates expression of MT3 in AML cells compared to mock transfected cells. (B) CCK-8 assays show that transfection with MT3 lentivirus inhibits proliferation in HL-60 and MV4-11 cells in a dose-dependent manner compared to mock transfected cells. (C) The number of cells displaying apoptotic features is higher in the HL-60 an MV4-11 cells transfected with PLVX-MT3compared to the mock transfected cells. (D) Quantification shows that the percentage of apoptotic cells in PLVX-MT3 transfected group is significantly higher compared to the percentage in the mock control group. *P < 0.05; **P < 0.01.Back to article page