Figure 2
The MT3 promoter is methylated in AML cell lines. (A) Four CpG island regions can be identified in the promoter of MT3. (B) MSP analysis of the methylation status of MT3 in leukemia cell lines shows that the promoter is hypermethylated in 7/11 cell lines. M and U represent MSP results using primer sets for methylated and unmethylated MT3 genes, respectively. (C) PCR analysis showed that methylation status of MT3 was decreased in leukemia cells following 5-Aza treatment compared to control cells treated with DMSO. The transcript level of MT3 is significantly upregulated in HL-60 cells and MV4-11cells treated with 5-Aza compared to those treated with DMSO. *P < 0.05; **P < 0.01.