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Table 3 Pre-specified hypotheses for testing in the current study cohort

From: High-dimensional analysis of the aging immune system: Verification of age-associated differences in immune signaling responses in healthy donors

Hypothesis order SCNP Node | Cell subset Wilcoxon p-value Hypothesis verified?
1 IL-2 → p-Stat5 | CD45RA+ Th cells 1.10 × 10-8 Yes
2 IFN-α → p-Stat5 | CD45RA+ cytotoxic T cells 5.65 × 10-10 Yes
3 IL-27 → p-Stat5 | CD45RA+ cytotoxic T cells 9.38 × 10-11 Yes
4 IFN-α → p-Stat1 | CD45RA+ cytotoxic T cells 8.67 × 10-8 Yes
5 IL-6 → p-Stat1 | CD45RA+ cytotoxic T cells 2.46 × 10-9 Yes
6 IL-4 → p-Stat6 | CD45RA+ cytotoxic T cells 1.86 × 10-4 Yes
7 IL-6 → p-Stat3 | CD45RA+ cytotoxic T cells 6.70 × 10-7 Yes
8 IFN-α → p-Stat5 | CD45RA+ Th cells 5.28 × 10-6 Yes
9 IL-27 → p-Stat1 | CD45RA+ cytotoxic T cells 7.70 × 10-8 Yes
10 IFN-α → p-Stat3 | CD45RA+ cytotoxic T cells 0.180 No
11 PMA → p-S6 | CD45RA+ cytotoxic T cells 4.82 × 10-6 No*
  1. *This age-association is significantly associated with age in the test cohort, but not verified according to the requirements of the gatekeeper approach. Hypotheses were sequentially ordered for testing using a Gatekeeper strategy. A p value < 0.05 for the two-sided Wilcoxon rank-sum test statistic was considered significant.