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Table 2 Signaling nodes measured

From: High-dimensional analysis of the aging immune system: Verification of age-associated differences in immune signaling responses in healthy donors

Signaling node Measured in prior study
IFN-α → p-Stat1 Yes
IFN-α → p-Stat3 Yes
IFN-α → p-Stat5 Yes
IFN-α → p-Stat6 Yes
IL-2 → p-Stat5 Yes
IL-2 → p-Stat6 Yes
IL-4 → p-Stat5 Yes
IL-4 → p-Stat6 Yes
IL-6 → p-Stat1 Yes
IL-6 → p-Stat3 Yes
IL-27 → p-Stat1 Yes
IL-27 → p-Stat3 Yes
IL-27 → p-Stat5 Yes
IL-27 → p-Stat6 Yes
R848 → p-Erk Yes
R848 → p-NF-κB Yes
PMA → p-S6 Yes
PMA → p-Erk Yes
Anti-IgD → p-S6 No
Anti-IgD → p-Erk No
Anti-IgM → p-ZAP70/p-Syk No
Anti-IgM → p-Erk No
Anti-CD3 → p-ZAP70/p-Syk No
Anti-CD3 → p-Erk No
  1. The following 24 signaling nodes (modulator and intracellular readout combinations) were measured in the current study. The majority of these signaling nodes (i.e. the 18 signaling nodes indicated above) were also measured in the previously published study cohort [7]. All signaling nodes were measured in 12 cell populations defined by their surface phenotypes including 7 distinct immune cell subpopulations (monocytes, B cells, CD3-CD20- lymphocytes (NK cell-enriched subpopulation), CD45RA+ Th cells, CD45RA- Th cells, CD45RA+ cytotoxic T cells, and CD45RA- cytotoxic T cells).