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Table 2 Signaling nodes measured

From: High-dimensional analysis of the aging immune system: Verification of age-associated differences in immune signaling responses in healthy donors

Signaling node

Measured in prior study

IFN-α → p-Stat1

Yes

IFN-α → p-Stat3

Yes

IFN-α → p-Stat5

Yes

IFN-α → p-Stat6

Yes

IL-2 → p-Stat5

Yes

IL-2 → p-Stat6

Yes

IL-4 → p-Stat5

Yes

IL-4 → p-Stat6

Yes

IL-6 → p-Stat1

Yes

IL-6 → p-Stat3

Yes

IL-27 → p-Stat1

Yes

IL-27 → p-Stat3

Yes

IL-27 → p-Stat5

Yes

IL-27 → p-Stat6

Yes

R848 → p-Erk

Yes

R848 → p-NF-κB

Yes

PMA → p-S6

Yes

PMA → p-Erk

Yes

Anti-IgD → p-S6

No

Anti-IgD → p-Erk

No

Anti-IgM → p-ZAP70/p-Syk

No

Anti-IgM → p-Erk

No

Anti-CD3 → p-ZAP70/p-Syk

No

Anti-CD3 → p-Erk

No

  1. The following 24 signaling nodes (modulator and intracellular readout combinations) were measured in the current study. The majority of these signaling nodes (i.e. the 18 signaling nodes indicated above) were also measured in the previously published study cohort [7]. All signaling nodes were measured in 12 cell populations defined by their surface phenotypes including 7 distinct immune cell subpopulations (monocytes, B cells, CD3-CD20- lymphocytes (NK cell-enriched subpopulation), CD45RA+ Th cells, CD45RA- Th cells, CD45RA+ cytotoxic T cells, and CD45RA- cytotoxic T cells).