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Figure 1 | Journal of Translational Medicine

Figure 1

From: Rapid molecular genetic diagnosis of hypertrophic cardiomyopathy by semiconductor sequencing

Figure 1

Semiconductor sequencing flow diagram. Amplicons of 30 HCM genes are amplified by using 20 ng input genomic DNA and two primer pools. The about 200 bp amplicons are end-polished, barcodes and adapters ligated, purified to become prepared libraries. Emulsion PCR is conducted by Ion One Touch to generate ISPs for semiconductor sequencing. ISP indicates Ion Sphere Particles.

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