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Figure 3 | Journal of Translational Medicine

Figure 3

From: Downregulation of PPARγ by miR-548d-5p suppresses the adipogenic differentiation of human bone marrow mesenchymal stem cells and enhances their osteogenic potential

Figure 3

PPARγ is a target of miR-548d-5p in hBMSCs. (a) The putative miR-548d-5p seed region in the PPARγ 3’-UTR. (b) Western blotting analysis of PPARγ expression in transfected cells. β-actin was used as a reference. Blank, nontransfected cells; NC, cells transfected with scrambled miR-548d-5p negative control; miR-548d-5p, cells transfected with miR-548d-5p agomir. (c) Luciferase activity determined 48 h after transfection. Control, scrambled miRNA; miR-548d-5p, miR-548d-5p agomir; pmirGLO-PPARγ-Wt, wild-type pmirGLO-PPARγ; pmirGLO-PPARγ-Mut, mutant pmirGLO-PPARγ. *P < 0.05 compared with the control group.

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