Overexpression miR-548d-5p suppresses dexamethasone-induced adipogenic differentiation of hBMSCs. Blank, non-transfected and non-induced cells; Control, non-transfected hBMSCs induced with dexamethasone; NC, cells transfected with scrambled miR-548d-5p negative control and induced with dexamethasone; miR-548d-5p, cells transfected with miR-548d-5p agomir and induced with dexamethasone. (a) Oil red O staining on day 14 of culture. (b) Mature miR-548d-5p expression levels after transfection, U6 was used as an internal control. (c) TG containing was measured at day 14. (d) The ALP activity in the Control and NC groups were significantly lower than in the Blank group (P < 0.05), but there were no significant differences between the miR-548d-5p and Blank groups (P > 0.05). (e) PPARγ, C/EBPα, Runx2, and OCN mRNA levels were detected by qRT-PCR analysis. The data were normalized to GAPDH. (f) PPARγ, C/EBPα, Runx2, and OCN protein levels were detected by western blotting analysis, β-actin was used as a reference.