Figure 1

Dexamethasone induces adipogenic differentiation of hBMSCs and downregulates miR-548d-5p expression. Blank, non-induced hBMSCs; Dexamethasone-induced, hBMSCs induced with dexamethasone. (a) Oil red O staining showing more adipocytes were detectable in the cytoplasm of the cells in the dexamethasone-induced group than the Blank group at day 14. (b) TG content was measured at day 14. (c) ALP activity was measured using ELISA at day 6. (d) Expression levels of miR-548d-5p in the Dexamethasone-induced group and Blank group were measured by qRT-PCR at day 7 and day 14. U6 was used as an internal control. (e,f) qRT-PCR analysis of Runx2, OCN, PPARγ, and C/EBPα. The data were normalized to GAPDH. (g,h) Runx2, OCN, PPARγ, and C/EBPα protein levels were detected by Western blotting analysis, β-actin was used as a reference.