Methylation of Wnt antagonists and DNMTs expression in AML cells. A, MSP analysis. MSP was performed using previously published primer pairs as described in Methods. M, methylated reaction; U, unmethylated reaction; pos, positive control, neg, negative control. The PCR product for the M reaction was 158 bp long and that for the unmethylated reaction was 165 bp long; MSP, methylation-specific polymerase chain reaction. B, Expression of DNMT3A and DNMT3B in AML cell lines. Expression of DNMT3A and 3B were assayed by a standard western blot method in AML cells without treatment as the control group and AML cells with the treatment of DAC for 24 h, 48 h, and 72 h, respectively.