Figure 5From: Sequential combination of decitabine and idarubicin synergistically enhances anti-leukemia effect followed by demethylating Wnt pathway inhibitor promoters and downregulating Wnt pathway nuclear target Methylation of Wnt antagonists and DNMTs expression in AML cells. A, MSP analysis. MSP was performed using previously published primer pairs as described in Methods. M, methylated reaction; U, unmethylated reaction; pos, positive control, neg, negative control. The PCR product for the M reaction was 158Â bp long and that for the unmethylated reaction was 165Â bp long; MSP, methylation-specific polymerase chain reaction. B, Expression of DNMT3A and DNMT3B in AML cell lines. Expression of DNMT3A and 3B were assayed by a standard western blot method in AML cells without treatment as the control group and AML cells with the treatment of DAC for 24Â h, 48Â h, and 72Â h, respectively.Back to article page