Measurement of total and phosphorylated Akt 1/2/3 in primary AML samples. A) Samples (n = 4) were treated with lambda phosphatase and electropherogram demonstrated decrease in phosphorylated forms using total Akt 1/2/3 antibody. Two representative samples are shown. β-2 Microglobulin was used as loading control. X-axis represents iso-electric pH and y-axis represents luminescence units. B) Primary AML patient samples were examined at baseline for total and phosphorylated forms of Akt 1/2/3 and expressed as percentage phosphorylated against sample number (n = 11).