Measurement of total and phosphorylated 4EBP1 in primary AML samples. A) Samples (n = 8) were treated with 500 nM AZD-8055 and electropherogram demonstrated decrease in phosphorylated forms using total 4EBP1 antibody. Two representative samples are shown. β-2 Microglobulin was used as loading control. X-axis represents iso-electric pH and y-axis represents luminescence units. B) Primary AML patient samples were examined at baseline for total and phosphorylated forms of 4EBP1 and expressed as percentage phosphorylated against sample number (n = 11).