Use of 4EBP1 assay to examine target inhibition within MV4-11 cell line. MV4-11 cells were treated with mTOR1/2 inhibitor AZD-8055 (25 – 1000 nM) for one hour and inhibition of 4EBP1 phosphorylation was noted using the NanoPro 1000 and Western blotting. A) Electropherogram shows the decrease in signal using both total and phospho-specific antibodies. B) β-2 Microglobulin was used as loading control. X-axis represents iso-electric pH and y-axis represents luminescence units. C) Western blotting confirms the same results with GAPDH as loading control.