Figure 1

In vitro characterisation of murine MSCs (mMSCs). Passage 3 mMSCs underwent successful differentiation down adipogenic (A), osteogenic (B) and chrondrogenic (C) lineages after 21 days in appropriate differentiation media. D) Representative colony-forming unit analysis. E) Immunophenotypic profile of passage 3 mMSCs are negative for haematopoietic markers CD11b and CD45, endothelial marker CD31 and positive for mesenchymal markers CD44 and Sca-1. The solid peak is corresponding isotype control. Scale bars = 100 μm in A and B, 200 μm in C.