Table 1 Oligonucleotide sequences
From: NF-κB-modulated miR-130a targets TNF-α in cervical cancer cells
Name | Sequence (5’-3’) |
|---|---|
NF-κB-sence | CGGAATTCGCCACCAGAATGGCAGAAGATGATC |
NF-κB-antisence | TGTCACTCGAGGCAATTTTGCCTTCTAGAGGTC |
NF-κB-siR-Top | GATCCCGCCTGAACAAATGTTTCATTTGGTCAAGAGCCAAATGAAACATTTGTTCAGGCTTTTTTGGAAA |
NF-κB-siR-Bot | AGCTTTTCCAAAAAAGCCTGAACAAATGTTTCATTTGGCTCTTGACCAAATGAAACATTTGTTCAGGCGG |
Pri-130a-sense | ATGGGATCCAGAGGGAGCCCGTGAGCTG |
Pri-130a-antisense | CGGAATTCGTATAACTAACAAGTGAGGCTACC |
ASO-miR-130a | AUGCCCUUUUAACAUUGCACUG |
ASO-NC | TGACTGTACTGAGACTCGACTG |
miR-130a-RT | GTCGTATCCAGTGCAGGGTCCGAGGTGCACTGGATACGACATGCCCT |
miR-130a-Forward | TGCGGCAGTGCAATGTTA |
U6-RT | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAAAATATGG |
U6- Forward | TGCGGGTGCTCGCTTCGGCAGC |
miR-Reverse primer | CCAGTGCAGGGTCCGAGGT |
TNF-α-3’UTR-sense | CGGGATCCGAAATTGACACAAGTGGACC |
TNF-α-3’UTR-antisense | CGGAATTCCTCCCAAATAAATACATTCATCTG |
TNF-α-3’UTR-mut-sense | CCCTCTATTTATGATACGAGTTGTGATTATTT |
TNF-α-3’UTR-mut-antisense | AAATAATCACAACTCGTATCATAAATAGAGGG |
TNF-α-sense | CGGAATTCGCCACCATGAGCACTGAAAGC |
TNF-α-antisense | CCCGCTCGAGGCCAGGGCAATGATCCCAAAG |
β-actin-sense | CGTGACATTAAGGAGAAGCTG |
β-actin-antisense | CTAGAAGCATTTGCGGTGGAC |
GAPDH-sense | GCGAATTCCGTGTCCCCACTGCCAACGTGTC |
GAPDH-antisense | GCTACTCGAGTTACTCCTTGGAGGCCATGTGG |