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Figure 2 | Journal of Translational Medicine

Figure 2

From: NF-κB-modulated miR-130a targets TNF-α in cervical cancer cells

Figure 2

miR-130a promotes the growth of human cervical cancer cells. (A) The effects of NF-κB on miR-130a expression. Real-time RT-PCR was used to evaluate miR-130a levels in HeLa and C33A cells transfected with the pcDNA3/NF-κB or pSilencer/shR-NF-κB plasmids. Total RNA was extracted 24 h post-transfection and used for reverse transcription and real-time PCR. U6 snRNA was used to normalize gene expression. (B) Real-time RT-PCR was performed to detect the miR-130a levels in the two cell lines treated with pri-miR-130a or ASO-miR-130a. U6 snRNA was used to normalize gene expression. (C and D) MTT assays were performed to determine the effects of miR-130a on cell viability. HeLa and C33A cells were detached from 24-well plates after transfection with pcDNA3/pri-miR-130a or a control vector and ASO-miR-130a or control oligonucleotides, and the relative cell growth was determined at 24, 48 and 72 h after seeding in 96-well plates. (E and F) The effects of miR-130a on cell proliferation were evaluated by a colony formation assay. Cells were detached from 24-well plates after transfection and seeded in 12-well plates. On the 14th day after seeding, the number of colonies was determined. (G and H) MTT and colony formation assays using by NF-κB in cells with miR-130a knockdown. Experiments were performed in triplicate. (*P < 0.05, **P < 0.01).

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