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Figure 1 | Journal of Translational Medicine

Figure 1

From: NF-κB-modulated miR-130a targets TNF-α in cervical cancer cells

Figure 1

NF-κB promotes the growth of human cervical cancer cells. (A and B) Western blot analysis was utilized to detect NF-κB levels in HeLa cells and C33A cells transfected with pcDNA3/NF-κB or pSilencer/shR-NF-κB. After transfecting both cell lines, cells were cultured for 48 h, and then lysed for 30 min with RIPA buffer at 4°C. The endogenous GAPDH expression levels were used to normalize protein expression. (C and D) The effects of NF-κB on cell viability was assessed by MTT assays. HeLa and C33A cells were detached from 24-well plates after transfection with pcDNA3/NF-κB and pSilencer/shR-NF-κB or their respective control vectors. Relative cell growth was assessed at 24, 48 and 72 h after seeding in 96-well plates. (E and F) The effects of NF-κB on colony formation. Cells were detached from 24-well plates after transfection and seeded in 12-well plates, and on the 14th day after seeding, the number of colonies was counted. Experiments were performed in triplicate. (*P < 0.05, **P < 0.01).

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