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Figure 1 | Journal of Translational Medicine

Figure 1

From: Synergistic effects of IL-4 and TNFα on the induction of B7-H1 in renal cell carcinoma cells inhibiting allogeneic T cell proliferation

Figure 1

Expression analysis of IL-4, TNFα and their receptors in RCC cell lines. A) Constitutive TNFα mRNA expression, but lack of IL-4 mRNA expression was determined by conventional qPCR in different RCC cell lines (Hal31RC, MZ1257RC, MZ2514RC, MZ1790RC, Hal161RC) and in PBMC, which served as positive control. Representative data of at least 3 different experiments are shown. B) TNFα secretion of different RCC cell lines TNFα secretion was determined in Hal31RC, MZ1257RC, MZ2514RC, MZ1790RC, Hal161RC using ELISA. Representative data out of at least 3 different experiments are shown. C) The IL-4Rα and TNFRI-α expression was determined by qPCR in the different RCC cell lines and in PBMC, which served as positive control. Representative data of at least 3 different experiments are shown. D) Flow cytometric analyses of IL-4R and TNFαRI demonstrated the constitutive expression of IL-4R and TNFRI on Hal31RC and Hal161RC. The results are expressed as histograms. Bold line: staining, thin line: control. TNFRI is stained with an anti-TNFRI-PE labeled antibody, bold line: staining, thin line: isotype control. Representative data of at least 3 different experiments are shown.

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