Figure 5

GOLPH3 regulated 5-FU-induced apoptosis via modulation of PARP cleavage. RKO and LoVo cells were transfected with pCMV-Myc-GOLPH3/pCMV-Myc (GOLPH3 over-expression, A and C) or GOLPH3 siRNA pool (siG-pool)/control siRNA (siControl) (GOLPH3 silencing, B and D) and then treated with indicated concentrations of 5-FU (0, 100, 200 μM) for 48 h. Western blot analysis showed the alteration of cleaved PARP and GOLPH3. Upper panels, one representative of three similar Western blot analysis. Lower panels, relative protein ratio were normalized to β-actin. All data are mean ± SD, *P < 0.05.